Applications
Paleogenetics

Next-generation sequencing (NGS) capture technologies have revolutionized genetic sequencing of ancient and degraded DNA.

By combining the scale of NGS with the focused power of hybridization-based target capture, sequencing efforts can highlight endogenous DNA regions that matter most, whether from host or microbial sources, and eliminate contaminating DNA from sources like exogenous bacteria and fungi. Together, these technologies have opened up genetic study to a wide range of plant, animal, and environmental sources that were previously out of reach, such as archaeological, paleoclimate, environmental, historical, museum, herbarium, and many more. Flexible, in-solution NGS capture with technology such as myBaits®, allows enrichment of even extremely rare, damaged, and short molecules (<50bp) that are impossible to assay with traditional PCR-based amplicon sequencing.

With myBaits® Capture Kits, targeted sequencing of even extinct animals for which a reference genome sequence is unavailable has become routine. Importantly, the adaptable nature of hybridization capture can tolerate unknown divergence between bait reference sequence and target molecules, which are frequently present in aDNA specimens due to both biological mutations as well as postmortem damage. myBaits® target capture solutions are perfect for any scale of genetic study; single loci such as mitochondrial DNA, hundreds to thousands of custom loci, and entire nuclear genomes.

The scientific team at Arbor Biosciences has extensive experience working with ancient DNA, and can offer detailed design and protocol advice to cater experiments to the unique features of degraded DNA samples. In addition, Arbor Biosciences offers in-house NGS services for ancient and degraded DNA samples in our dedicated anti-contamination facilities, where our team of experts utilizes the latest library preparation chemistry tailored for low-input, degraded nucleic acids.

Featured Publications

D.I. Cruz-Davalos et al. (2017). Experimental conditions improving in-solution target enrichment for ancient DNA. Molecular Ecology Resources

A. Grealy et al. (2017). Eggshell palaeogenomics: Palaeognath evolutionary history revealed through ancient nuclear and mitochondrial DNA from Madagascan elephant bird (Aepyornis sp.) eggshell. Molecular Phylogenetics and Evolution

A.M. Devault et al. (2017). A molecular portrait of maternal sepsis from Byzantine Troy. elife

J.M. Enk et al. (2016). Mammuthus Population Dynamics in Late Pleistocene North America: Divergence, Phylogeography, and Introgression. Frontiers in Ecology and Evolution

P.D. Heintzman et al. (2016). Bison phylogeography constrains dispersal and viability of the Ice Free Corridor in western Canada. PNAS

A.T. Duggan et al. (2016). 17th Century Variola Virus Reveals the Recent History of Smallpox. Current Biology

J.M. Enk et al. (2014). Ancient Whole Genome Enrichment Using Baits Built from Modern DNA. Molecular Biology and Evolution

Team Expertise