Products
myBaits® Expert Panels – Plant Universal – Angiosperms

The new Angiosperms353 panel enriches 100's of single-copy genes orthologous across all angiosperms (flowering plants).

Combining the flexible hybridization power of in-solution target capture with an expertly selected set of orthologous locus sequences, this new probe set has been demonstrated to enrich hundreds of putatively single-copy protein-coding genes across a broad range of angiosperms (flowering plants). Probes were designed from 353 loci, each with 5-15 representative sequences from across all angiosperms which were selected using a novel “k-medoids clustering approach” to maximize taxonomic breadth of the design (Johnson et al 2018, bioRxiv).

This baitset is broadly applicable for phylogenetic research across all flowering plants, and is available from Arbor Biosciences as an in-stock catalog kit available for immediate shipment at a low per-reaction cost. As with all myBaits kits, the Angiosperms353 panel is provided as a complete solution target capture kit, including buffers, blockers, and baits, along with an easy-to-use protocol. Or if you would prefer to outsource the work, our myReads NGS service expert scientists are available to perform library preparation, target capture, and sequencing for your entire project.

Learn more:

M Johnson, L Pokorny, S Dodsworth, LR Botigue, RS Cowan, A Devault, WL Eiserhardt, N Epitawalage, F Forest, JT Kim, JHH Leebens-Mack, IJ Leitch, O Maurin, DE Soltis, PS Soltis, GK Wong, WJ Baker, N Wickett. (2018). “A Universal Probe Set for Targeted Sequencing of 353 Nuclear Genes from Any Flowering Plant Designed Using k-medoids Clustering“. bioRxiv 361618; doi: https://doi.org/10.1101/361618

GitHub: “HybSeq Target Capture of 353 Protein Coding Genes in Any Angiosperm



Fig 1.Heatmap of Gene Recovery Efficiency. Each row is one sample, and each column is one gene. Colors indicate the percentage of the target length (calculated by the mean length of all k-medoid transcripts for each gene) recovered.” (pg 19, Johnson et al 2018, bioRxiv). Image is Figure 3 from Johnson et al 2018, bioRxiv.


Fig 2.Total Length of Sequence Recovery for Both Coding and Non-coding Regions Across 353 Loci for 42 Angiosperm Species. Reads were mapped back to either coding sequence (yellow) or coding sequence plus flanking non-coding (i.e. intron) sequence (purple)… The total length of coding sequence targeted was 260,802 bp. The median recovery of coding sequence was 137,046 bp and the median amount of non-coding sequence recovered was 216,816 bp (with at least 8x depth of coverage).” (pg 21, Johnson et al 2018, bioRxiv). Image is modified version of Figure 4 from Johnson et al 2018, bioRxiv.

Features & Benefits

Predesigned panel
Designed by expert plant geneticists
Proven results
Demonstrated utility across broad taxonomic range
Freely accessible pipelines
Simple, user-friendly data analysis
Cost-effective
Use one bait set across multiple distantly-related taxa

Current Version: v4

For kits received starting in April 2018

Your kit has Arbor Biosciences packaging.
myBaits-Manual-v4.pdf

Ordering Information

Catalog # Description Quantity Price Contact Us
308108 myBaits Angiosperms 353 v1 8 rxns 8 reactions $1000 Order Now
308148 myBaits Angiosperms 353 v1 48 rxns 48 reactions $3610 Order Now
308196 myBaits Angiosperms 353 v1 96 rxns 96 reactions $4980 Order Now

Team Expertise