Products
myTXTL® – Toolbox 2.0 Plasmid Collection

myTXTL® Toolbox 2.0 Plasmid Collection is an excellent source for designing gene circuits.

The combination of synthetic gene networks with in vitroprotein production technology opens new innovative fields of application in medicine and biotechnology. Examples are environmental sensors, controls for biomanufacturing of biofuels, stem cell medicine, gene therapy (CRISPR), and manufacturing of functional materials. Cell-free platforms help to overcome limitations occurring in living host organisms, which are typically tied to the biochemical cross-talk between the host cell and the artificial gene circuit and a consequence of the potentially cytotoxic effect of the exogeneous gene network on the living cell on the one hand, and the availability of resources for transcription and translation, DNA replication and metabolites on the other. As those resources are dependent on the cell density, growth rate and cultivation conditions, deploying cell-free systems for gene circuit testing creates a more controlled environment thus increasing reproducibility and robustness of the circuit behavior and output.

Gene Circuits

The myTXTL® Toolbox 2.0 Plasmid Collection was designed and intensively studied by Vincent Noireaux, PhD at the University of Minnesota and co-workers. It contains over one hundred plasmids with various promoters and open reading frames (ORFs) to investigate gene regulation and molecule turnover. Available open reading frames include a wide selection of transcription factors, TXTL modulators and fluorescent reporter proteins to build multi-stage gene circuits.

A gene circuit executed in any myTXTL® kit is required to start with a σ70-specific promoter, as gene expression in myTXTL® relies entirely on the endogenous TXTL machinery of E. coli. Its modular and pre-designed format makes it also ideal for educational purposes to train students and demonstrate basic concepts of synthetic biology.

Gene Synthesis

In addition to the listed plasmids, Arbor Biosciences’ DNA synthesis service provides high-quality, error-free DNA tailored to your experimental needs and optimized for in vitro protein expression. For more information, please visit our myDNA® product page.

Ordering Information

Catalog # Description Quantity Price Contact Us
502002 pTXTL-P19a-deCFP 1 µg $65 Order Now
502003 pTXTL-P19a-deYFP 1 µg $65 Order Now
502004 pTXTL-P19a-ntrC 1 µg $65 Order Now
502005 pTXTL-P19a-S24 1 µg $65 Order Now
502006 pTXTL-P19a-ompA-S24 1 µg $65 Order Now
502007 pTXTL-P19a-S28 1 µg $65 Order Now
502008 pTXTL-P19a-S28-ssrA 1 µg $65 Order Now
502009 pTXTL-P19a-S38 1 µg $65 Order Now
502010 pTXTL-P24a-deGFP 1 µg $65 Order Now
502011 pTXTL-P24a-S28 1 µg $65 Order Now
502012 pTXTL-P24a-ompA-S38 1 µg $65 Order Now
502013 pTXTL-P24a-S28-ssrA 1 µg $65 Order Now
502014 pTXTL-P24a-S38 1 µg $65 Order Now
502015 pTXTL-P28a-aH 1 µg $65 Order Now
502017 pTXTL-P28a-cI-ssrA 1 µg $65 Order Now
502019 pTXTL-P28a-deGFP 1 µg $65 Order Now
502020 pTXTL-P28a-deGFP-ssrA 1 µg $65 Order Now
502021 pTXTL-P28a-deYFP 1 µg $65 Order Now
502022 pTXTL-P28a-flgM 1 µg $65 Order Now
502023 pTXTL-P28a-flgM-ssrA 1 µg $65 Order Now
502025 pTXTL-P28a-mApple 1 µg $65 Order Now
502027 pTXTL-P28a-mazE-ssrA 1 µg $65 Order Now
502028 pTXTL-P28a-mreB 1 µg $65 Order Now
502029 pTXTL-P28a-mreC 1 µg $65 Order Now
502030 pTXTL-P28a-ntrC 1 µg $65 Order Now
502031 pTXTL-P28a-S19 1 µg $65 Order Now
502032 pTXTL-P28a-S19-ssA 1 µg $65 Order Now
502033 pTXTL-P28a-S24-ssrA 1 µg $65 Order Now
502034 pTXTL-P28a-ompA-S24-ssrA 1 µg $65 Order Now
502035 pTXTL-P28a-S38 1 µg $65 Order Now
502036 pTXTL-P28a-S54 1 µg $65 Order Now
502037 pTXTL-P28a-tetR 1 µg $65 Order Now
502038 pTXTL-P28a-venus-mreB 1 µg $65 Order Now
502039 pTXTL-P28a-venus-mreB-18L 1 µg $65 Order Now
502040 pTXTL-P32a-deGFP 1 µg $65 Order Now
502041 pTXTL-P38a-deGFP 1 µg $65 Order Now
502042 pTXTL-P38a-ntrC 1 µg $65 Order Now
502043 pTXTL-P38a-S19 1 µg $65 Order Now
502044 pTXTL-P38a-S19-ssrA 1 µg $65 Order Now
502045 pTXTL-P38a-S28 1 µg $65 Order Now
502046 pTXTL-P38a-S28-ssrA 1 µg $65 Order Now
502047 pTXTL-P38a-S54 1 µg $65 Order Now
502048 pTXTL-P54a-deGFP 1 µg $65 Order Now
502049 pTXTL-P54a-S19 1 µg $65 Order Now
502050 pTXTL-P54a-S24 1 µg $65 Order Now
502051 pTXTL-P54a-S28 1 µg $65 Order Now
502052 pTXTL-P54a-S38 1 µg $65 Order Now
502053 pTXTL-P70a-clpXP 1 µg $65 Order Now
502054 pTXTL-P70a-cI-ssrA 1 µg $65 Order Now
502055 pTXTL-P70a-deCFP 1 µg $65 Order Now
502056 pTXTL-P70a-deGFP 1 µg $65 Order Now
502057 pTXTL-P70a-deGFP-ssrA 1 µg $65 Order Now
502058 pTXTL-P70a-deYFP 1 µg $65 Order Now
502059 pTXTL-P70a-dmVenus 1 µg $65 Order Now
502060 pTXTL-P70a-dTomato 1 µg $65 Order Now
502061 pTXTL-P70a-mApple 1 µg $65 Order Now
502063 pTXTL-P70a-mmCherry 1 µg $65 Order Now
502064 pTXTL-P70a-mGapt1 1 µg $65 Order Now
502065 pTXTL-P70a-mGapt-deGFP 1 µg $65 Order Now
502066 pTXTL-P70a-mRuby 1 µg $65 Order Now
502067 pTXTL-P70a-S19 1 µg $65 Order Now
502068 pTXTL-P70a-S19-ssrA 1 µg $65 Order Now
502069 pTXTL-P70a-S24 1 µg $65 Order Now
502071 pTXTL-P70a-ntrC 1 µg $65 Order Now
502072 pTXTL-P70a-S28 1 µg $65 Order Now
502073 pTXTL-P70a-S28-ssrA 1 µg $65 Order Now
502075 pTXTL-P70a-S32 1 µg $65 Order Now
502076 pTXTL-P70a-S32-ssrA 1 µg $65 Order Now
502077 pTXTL-P70a-S38 1 µg $65 Order Now
502078 pTXTL-P70a-ompA-S38 1 µg $65 Order Now
502079 pTXTL-P70a-S54 1 µg $65 Order Now
502081 pTXTL-P70a-T3rnap 1 µg $65 Order Now
502082 pTXTL-P70a-T7rnap 1 µg $65 Order Now
502083 pTXTL-P70a-U3-deGFP 1 µg $65 Order Now
502084 pTXTL-P70a-U4-deGFP 1 µg $65 Order Now
502085 pTXTL-P70a-U5-deGFP 1 µg $65 Order Now
502086 pTXTL-P70a-venus 1 µg $65 Order Now
502087 pTXTL-P70b-deGFP 1 µg $65 Order Now
502088 pTXTL-P70b-mGapt1 1 µg $65 Order Now
502089 pTXTL-P70b-mGapt-deGFP 1 µg $65 Order Now
502090 pTXTL-P70b-U3-deGFP 1 µg $65 Order Now
502091 pTXTL-P70b-U4-deGFP 1 µg $65 Order Now
502092 pTXTL-P70c-deGFP 1 µg $65 Order Now
502093 pTXTL-P70c-mGapt1 1 µg $65 Order Now
502094 pTXTL-P70c-mGapt-deGFP 1 µg $65 Order Now
502095 pTXTL-P70c-U3-deGFP 1 µg $65 Order Now
502096 pTXTL-P70c-U4-deGFP 1 µg $65 Order Now
502097 pTXTL-P70d-deGFP 1 µg $65 Order Now
502098 pTXTL-PLtet01-deGFP 1 µg $65 Order Now
502099 pTXTL-PLtet01-deGFP-ssrA 1 µg $65 Order Now
502100 pTXTL-PLtet01-deGFP-YbaQ 1 µg $65 Order Now
502102 pTXTL-pC-mGapt1 1 µg $65 Order Now
502103 pTXTL-pCa-deGFP 1 µg $65 Order Now
502104 pTXTL-T3P-deGFP 1 µg $65 Order Now
502105 pTXTL-T7p7-deGFP 1 µg $65 Order Now
502106 pTXTL-T7p7-mGapt 1 µg $65 Order Now
502107 pTXTL-T7p8-deGFP 1 µg $65 Order Now
502108 pTXTL-T7p8-mGapt 1 µg $65 Order Now
502109 pTXTL-T7p11-deGFP 1 µg $65 Order Now
502110 pTXTL-T7p11-mGapt 1 µg $65 Order Now
502111 pTXTL-T7p14-deGFP 1 µg $65 Order Now
502112 pTXTL-T7p14-mGapt 1 µg $65 Order Now
502113 pTXTL-T7p14-aH 1 µg $65 Order Now
502114 pTXTL-T7p14-aH-eGFP 1 µg $65 Order Now
502115 pTXTL-T7p16-deGFP 1 µg $65 Order Now
502116 pTXTL-T7p16-mGapt 1 µg $65 Order Now