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myBaits® Onconome v2 – Target Capture Kit for Oncology Research

The myBaits Onconome v2 NGS capture kit targets thousands of exons in hundreds of cancer-related genes.

These genes were described in two major studies: Kandoth et al 2013 “Mutational landscape and significance across 12 major cancer types” (Nature 502:333-339, doi:10.1038/nature12634) and Lawrence et al 2014 “Discovery and saturation analysis of cancer genes across 21 tumour types” (Nature 505:495-501, doi: 10.1038/nature12912). The panel targets ~1.5Mb of total sequence from 290 genes indentified between the two studies for their roles in cancer.

The Onconome v2 panel was optimized through multiple design iterations in order to improve coverage uniformity and filter out repetitive genomic loci. Many of the selected loci are implicated in a variety of human phenotypes beyond just cancer. The panel is an excellent, cost-effective choice for exploratory targeted exome sequencing in a variety of research contexts. myBaits kits are provided as a complete solution for targeted sequencing including buffers, bait sets and blockers specific to the NGS library prep of choice. All myBaits kits are for research-use only, and are not authorized or validated for diagnostic or therapeutic purposes.

The Onconome v2 panel can be customized by adding or removing specific loci from the bait set through the myBaits Custom panel service. Additionally, our team of scientific experts can help design a custom kit for targeting non-cancer related genes, and other human genomic DNA targets to meet a specific research need. Contact us to learn more about maximizing the efficiency of any NGS study by targeting only specific markers or genes of interest.

Features & Benefits

Predesigned panel
Optimized performance for oncology research
Wide coverage
PanCancer panel targeting 1000’s of exons from 100’s of genes
Curated targets
Loci selected from major cancer field publications
Cost-effective
Lower per-reaction price than whole genome or exome sequencing

Figure 1. With US$15 in per-sample sequencing, percentage of targeted positions with at least 10X, 20X, and 30X unique read depth. >95% of intended positions have at least 10X read depth with minimal sequencing cost expenditure, providing the user with extremely cost-effective and focused performance from the myBaits Onconome v2 panel.

 

 

Figure 2. Example visualizing NGS reads from enriched human gDNA library, aligned to the hg38 genomic reference sequence. Positions of the four myBaits OnconomeV2 probes in this region are indicated by blue bars. Unique read coverage is highest across the baited region, and tapers off upstream and downstream of the baited region, facilitating highly efficient use of NGS.

 

 

Table 1. Genes included in Onconome v2 panel

  • ACO1
  • BRCA1
  • CUX1
  • FOXA1
  • KEL
  • MYOCD
  • PPP2R1A
  • SIRT4
  • TNFRSF14
  • ACVR1B
  • BRCA2
  • DDX3X
  • FOXA2
  • KIT
  • NAV3
  • PPP6C
  • SLC1A3
  • TP53
  • ACVR2A
  • BRE
  • DDX5
  • FRMD7
  • KLHL8
  • NBPF1
  • PRDM1
  • SLC26A3
  • TP53BP1
  • ACVR2B
  • CAP2
  • DIAPH1
  • GATA3
  • KMT2A
  • NCOR1
  • PRX
  • SLC44A3
  • TPX2
  • ADNP
  • CARD11
  • DIS3
  • GNA13
  • KMT2B
  • NF1
  • PTEN
  • SLC4A5
  • TRAF3
  • AJUBA
  • CASP8
  • DNAH12
  • GNB1
  • KMT2C
  • NFE2L2
  • PTPN11
  • SMAD2
  • TRIM23
  • AKT1
  • CBFB
  • DNER
  • GNPTAB
  • KMT2D
  • NFE2L3
  • QKI
  • SMAD4
  • TSC1
  • ALK
  • CCDC120
  • DNMT3A
  • GOT1
  • KRAS
  • NOTCH1
  • RAB40A
  • SMARCA4
  • TSHZ2
  • ALKBH6
  • CCDC6
  • EGFR
  • GPS2
  • LCTL
  • NPM1
  • RAC1
  • SMARCB1
  • TSHZ3
  • ALPK2
  • CCND1
  • EGR3
  • GUSB
  • LIFR
  • NRAS
  • RAD21
  • SMC1A
  • TTLL9
  • ANK3
  • CD1D
  • EIF2S2
  • H3F3C
  • LRRK2
  • NSD1
  • RASA1
  • SMC3
  • TXNDC8
  • APC
  • CD70
  • EIF4A2
  • HGF
  • MALAT1
  • NTN4
  • RB1
  • SNX25
  • U2AF1
  • APOL2
  • CD79B
  • ELF3
  • HIST1H1C
  • MAP2K1
  • NUP210L
  • RBM10
  • SOS1
  • USP9X
  • AR
  • CDC27
  • EP300
  • HIST1H1E
  • MAP2K4
  • ODAM
  • RHEB
  • SOX17
  • VEZF1
  • ARHGAP35
  • CDH1
  • EPHA2
  • HIST1H2BD
  • MAP3K1
  • OMA1
  • RHOA
  • SOX9
  • VHL
  • ARID1A
  • CDK12
  • EPHA3
  • HIST1H3B
  • MAP4K3
  • OTUD7A
  • RIT1
  • SPEN
  • WASF3
  • ARID2
  • CDK4
  • EPHB6
  • HIST1H4E
  • MAPK8IP1
  • PAPD5
  • RPL22
  • SPOP
  • WT1
  • ARID5B
  • CDKN1A
  • ERBB2
  • HRAS
  • MBD1
  • PBRM1
  • RPL5
  • SRSF2
  • XIRP2
  • ASXL1
  • CDKN1B
  • ERBB3
  • HSP90AB1
  • MECOM
  • PCBP1
  • RPS15
  • STAG2
  • XPO1
  • ASXL2
  • CDKN2A
  • ERBB4
  • IDH1
  • MED12
  • PDAP1
  • RPS2
  • STK11
  • ZFHX3
  • ATM
  • CDKN2C
  • ERCC2
  • IDH2
  • MED23
  • PDCD2L
  • RSBN1L
  • STX2
  • ZNF180
  • ATP5B
  • CEP76
  • EZH1
  • IL7R
  • MET
  • PDGFRA
  • RUNX1
  • TAF1
  • ZNF471
  • ATR
  • CHD4
  • EZH2
  • ING1
  • MGA
  • PDSS2
  • RXRA
  • TBC1D12
  • ZNF483
  • ATRX
  • CHD8
  • EZR
  • INPPL1
  • MICALCL
  • PHF6
  • SACS
  • TBL1XR1
  • ZNF620
  • AXIN2
  • CHEK2
  • FAM166A
  • INTS12
  • MIR142
  • PIK3CA
  • SELP
  • TBX3
  • ZNF750
  • AZGP1
  • CNBD1
  • FAM46C
  • IPO7
  • MORC4
  • PIK3CG
  • SEPT12
  • TCEB1
  • ZRANB3
  • B2M
  • CNKSR1
  • FAT1
  • IRF4
  • MPO
  • PIK3R1
  • SERPINB13
  • TCF7L2
  • B4GALT3
  • COL5A1
  • FBXW7
  • IRF6
  • MTOR
  • PLCG2
  • SETBP1
  • TCP11L2
  • BAP1
  • COL5A3
  • FGFBP1
  • ITGB7
  • MUC17
  • POLE
  • SETD2
  • TDRD10
  • BCLAF1
  • CREBBP
  • FGFR2
  • ITPKB
  • MXRA5
  • POLQ
  • SETDB1
  • TET2
  • BCOR
  • CTCF
  • FGFR3
  • KDM5C
  • MYB
  • POU2AF1
  • SF3B1
  • TGFBR2
  • BHMT2
  • CTNNB1
  • FLG
  • KDM6A
  • MYCN
  • POU2F2
  • SGK1
  • TIMM17A
  • BRAF
  • CUL4B
  • FLT3
  • KEAP1
  • MYD88
  • PPM1D
  • SIN3A
  • TLR4

Ordering Information

Catalog # Description Quantity Price Contact Us
304016 myBaits Onconome v2.0 16 $1680 Order Now
304048 myBaits Onconome v2.0 48 $3360 Order Now
304096 myBaits Onconome v2.0 96 $5760 Order Now

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